Molecular Characterization and Antimicrobial Resistance Profiling of Extended Spectrum Beta-lactamase (ESBL) Producing Escherichia coli in Bovines from J and K, India

Background: Higher prevalence of ESBL producers is alarming in dairy sector. So limiting antimicrobial use may curtail the selection and persistence predominant conjugative plasmids among strains. The study was aimed to determine occurrence extended-spectrum beta-lactamase (ESBL) producing E. coli as well their genetic diversity, resistance integrons bovines. Methods: A total 180 faecal samples were screened for presumptive isolates. All isolates subjected screening test confirmed by double disc. Further, these tested presence bla genes those found positive multidrug (MDR) disk diffsion. MDR further intI 1, 2 3 genes. Result: 360 obtained. Out which, 154 (42.77%) be resistant producers. Of isolates, 120 (77.92%) carried gene/s blaTEM, blaCTX-M blaSHV are declared multi drug resistant. 59 integron gene.